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产品目录

PKM2/PKM specific抗体图片
产品货号:
Z24063
中文名称:
PKM2/PKM specific抗体
英文名称:
Anti-PKM2/PKM specific antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称Anti-PKM2/PKM specific antibody
指标别名CTHBP;OIP 3;OIP3;Opa interacting protein 3;p58;PK2;PK3;PKM;PKM2;PK-M2;PKM2 pyruvate kinase;muscle;Pyruvate kinase 2/3;Pyruvate kinase isozymes M1/M2;Pyruvate kinase muscle isozyme;TCB;THBP1;Tumor M2 PK
克隆性Polyclonal
检验物种human,mouse
应用范围WB,IHC,ICC/IF,IP,Direct ELISA
基因名称PKM
抗体来源Rabbit
抗体类型IgG
免疫原Peptide
实际分子量58KD
成分500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol.
纯化方式Immunogen affinity purified.
浓度500μg/mL
产品形态溶液
保存条件12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
背景资料PKM (Pyruvate Kinase,Muscle),also known as PK3 or PKM2,is an enzyme that in humans is encoded by the PKM gene.The activity of pyruvate kinase subtype M2 is increased by fructose 1,6-bisphosphate (Fru-1,6-P2).By in situ hybridization,Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25.Ashizawa et al.(1991) manipulated the intracellular Fru-1,6-P2 concentration in several mammalian cell lines,including human,by varying the glucose concentration in the media.Using a novel proteomic screen for phosphotyrosine-binding proteins,Christofk et al.(2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.
Uniprot IDP14618
推荐配套试剂Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P) and ICC.
基因名全称pyruvate kinase,muscle
蛋白名全称Pyruvate kinase PKM
推荐稀释比
Western blot (WB) 1:500~2000
Immunohistochemistry in paraffin section (IHC) 1:50~400
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50~400
ImmunoPrecipitation (IP) 1:50
Direct ELISA 1:100~1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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